Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages
ABSTRACT Staphylococcus aureus is a prominent global nosocomial and community-acquired bacterial pathogen.A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates of S.aureus.Here, we describe the construction and characterization of the IMXXB series of Escherichia coli strains that mimic t